Fig. 4: Effect of TGFβR1 inhibition on aged hematopoiesis. | Nature Communications

Fig. 4: Effect of TGFβR1 inhibition on aged hematopoiesis.

From: Micro-environmental sensing by bone marrow stroma identifies IL-6 and TGFβ1 as regulators of hematopoietic ageing

Fig. 4: Effect of TGFβR1 inhibition on aged hematopoiesis.The alternative text for this image may have been generated using AI.

Bar graphs showing the number of LT-HSCs (a), LSK (b) and LK (c) cells in bone marrow of old mice treated with vehicle (VEH) or SB (LT-HSCs and LSK: N = 7/condition; LK: VEH N = 6, SB N = 7) as frequency of live single cells. Data are from three independent experiments. Values show mean ± s.e.m. *P < 0.05; ***P < 0.001 (two-tailed unpaired Student’s t test). Exact P values: LT-HSCs: 0.00000072; LSK: 0.0200; LK: 0.011. d Representative flow cytometry plots of LT-HSC cell cycle distribution from vehicle and SB-treated old mice. Gating for G0, G1 and S/G2/M cell cycle phases is shown, as are the P values for comparison between the two conditions. e Quantification of LT-HSCs in each phase of the cell cycle from vehicle (VEH) and SB-treated old mice (VEH: N = 6; SB: N = 8; three independent experiments). Values are mean ± s.d. **P < 0.01 (two-tailed unpaired Student’s t test). Exact P values: G0: 0.0092; G1: 0.0072; S/G2/M: 0.1206. f Representative flow cytometry plots of myelo-erythroid progenitors from vehicle (top panels) and SB-treated old mice (bottom panels). g Quantification of myelo-erythroid progenitors from vehicle (VEH) and SB-treated old mice (VEH: N = 6; SB: N = 7; three independent experiments). Values are mean ± s.e.m. *P < 0.05; ***P < 0.001 (two-tailed unpaired Student’s t test). Exact P values: MkP: 0.000000005; preGM: 0.0007; GMP: 0.00000001; preMegE: 0.00000024; preCFU-E: 0.0000000033; CFU-E: 0.0148. h PB platelet count in vehicle (VEH) and SB-treated old mice (VEH: N = 14, SB: N = 16). Data are from seven independent experiments. Values are mean ± s.e.m. **P < 0.01; (two-tailed unpaired Student’s t test). Exact P value: 0.0026. Source data are provided as a source data file.

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