Fig. 1: CRISPR GUARD protection of Cas9 off-target sites.
From: CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs
Schematic showing how short gRNAs (orange) forming catalytically inactive complexes with Cas9 can occupy specific off-target sites in the genome and compete with the mismatched nuclease competent gRNA (black), thereby providing protection from Cas9-mediated DNA cleavage. Mismatches in the gRNA are shown in red. RNA bases overlapping the gRNA PAM are shown in bold.
