Fig. 2: Formation of peptide-type nanofibers and hydrogel through an oxime formation reaction.
From: Control of seed formation allows two distinct self-sorting patterns of supramolecular nanofibers
a Schematic illustration of the nanofiber formation (sol–gel transition) through oxime formation. b Macroscopic sol–gel transition of Ald-F(F)F upon (top) O-benzylhydroxylamine and (bottom) buffer treatment. c Rheological properties of the hydrogel. G’: storage shear modulus, G’’: loss shear modulus. Frequency: 10 rad/s, strain amplitude: 1%. d HPLC analysis of the formation rate of BnOx-F(F)F. Internal standard: fluorescein. e High-resolution Airyscan CLSM images (left) before and 1 h after addition of (middle) O-benzylhydroxylamine or (right) buffer to Ald-F(F)F. Condition: [Ald-F(F)F] = 4.3 mM (0.20 wt%), [O-benzylhydroxylamine] = 4.3 mM (1.0 eq), [NP-Alexa647] = 4.0 µM, 100 mM MES, pH 6.0.
