Fig. 3: Characterization of the glycans.
From: Chemical synthesis of glycans up to a 128-mer relevant to the O-antigen of Bacteroides vulgatus
∣ a Overlaid 1H-NMR spectra (acquired on Bruker 600 MHz with cryogenic probe and analyzed with Bruker Topspin) of the free glycans 1–7. b MALDI-FT-ICR-MS spectrum of 128-mer 7. The observed average m/z of 128-mer 7, detected as [M + Na]+, is in agreement with the calculated theoretical value (m/z 19771.1). c 2D diffusion ordered spectroscopy (DOSY) NMR spectra of glycans 1–7. The x-axis represents the 1H-NMR dimension; the y-axis represents the diffusion dimension; the double-logarithmic plot of D against Mw (Supplementary Fig. 13) provided a calibration curve described by the least-squares fitted linear equation Log D = −7873 − (0.486*Log Mw). Source data are provided as a Source Data file.
