Fig. 1: A genetically encoded sensor for BCM bioactivity.
From: Continuous bioactivity-dependent evolution of an antibiotic biosynthetic pathway
a Schematic representation of plasmids for BCM production and BCM-dependent GFP expression, featuring a constitutive promoter interrupted by the Rho-dependent λtR1 terminator. BCM biosynthesis from a plasmid carrying an anhydrotetracycline (aTC) inducible promoter results in GFP production by alleviating Rho-dependent transcriptional termination of GFP on reporter plasmid pREP.1. b GFP reporter pREP.1 response across a wide range of BCM concentrations. c GFP production upon induction of the P. aeruginosa wild-type (WT) or inactivated (S46A) BCM operon. All data shown as mean ± s.d.; n = 3 biological replicates. Source data underlying b, c are available as a Source Data file.
