Fig. 3: BCM efflux during PACE yields a more productive population of BGCs.

a Fluorescence (GFP) signal from BCM reporter plasmid pREP.2 in E. coli cells carrying the IPTG-inducible BCM efflux pump expression plasmid pPUMP and evolved BCM production plasmid pBCM.1 (recovered PACE clone 72.5). Results are absorbance normalized signal over uninduced controls, shown as mean ± s.d.; n = 3 biological replicates. b SP_BCM titers (plaque forming units, PFU) over an additional 72 h of PACE, using S2060 E. coli carrying accessory plasmid pAP.1 and the IPTG-inducible efflux pump expression plasmid pPUMP (induced with 5 μM IPTG), along with 15 μg mL⁻¹ MNNG for mutagenesis. Lagoon flow rate was maintained at 1 vol h⁻¹. c Summary and relative abundance of mutations observed in BCM operons following PACE with active BCM efflux (Pump PACE) from 24 sequenced operons (Supplementary Fig. 14). d. Summary of assayed BCM operon populations recovered from PACE (Supplementary Figs. 6, 7, and 14). The median value is marked with a line in each violin plot. Source data underlying a, b, d are available as a Source Data file.