Fig. 3: Genetic analyses of the Taf14–Sth1 interaction.
a Spotting assays with sth1Δ strains transformed with plasmids expressing full-length wild-type Sth1 or M4 mutant (L1204A/V1206A/I1208A/L1210A) at different temperatures. b Spotting assays with sth1Δ strains transformed with plasmids expressing full-length wild-type Sth1 or M4 mutant at different NaCl concentrations at 30 °C. c Spotting assays with sth1Δ strains transformed with plasmids expressing full-length wild-type or M4 mutant STH1 on plates containing hydroxyurea (HU), methyl methanesulfonate (MMS), and phleomycin (Phle) at two different concentrations at 30 °C. d Scatter plot of log10 normalized gene counts in the STH1 and sth1M4 strains. 108 genes were significantly (>2-fold change) upregulated (orange) and 202 genes were downregulated (blue) in the sth1M4 strain. e The qPCR validation of the RNA-seq results. Tir4, YML045W, and Hlr1 were upregulated, while Hsp30, Tps1, and Gpx1 were downregulated in sth1M4 strains. The Mgm1 transcription level is not regulated by Sth1. The change levels from RNA-seq were shown. Error bars represent standard deviations of four replicates. The relative expression level of wild-type STH1 strain genes was set to 1. * for P < 0.05; ** for P < 0.01; *** for P < 0.001; **** for P < 0.0001; two-tailed Student’s t-test. Data are presented as mean ± SD, n = 4. Source data are provided as a Source Data file. f Gene ontology analysis of the upregulated and downregulated differentially expressed genes. g Spotting assays of sth1Δ strains transformed with plasmids expressing full-length STH1 or sth1M4 on plates with galactose or raffinose as the sole carbon source at 30 °C.
