Fig. 3: Presynaptic t-LTP requires metabotropic glutamate receptors but not NMDARs.
From: Astrocyte-mediated switch in spike timing-dependent plasticity during hippocampal development
a Addition of D-AP5 (50 µm) to the superfusion fluid did not prevent t-LTP induction. The EPSP slope is shown in D-AP5-treated (red triangles, n = 6) and untreated cells (black triangles, n = 6). Bath-applied MK-801 (500 µm–1 mm) did not block the induction of t-LTP (gray triangles, n = 6). The traces show the EPSP before (1) and 30 min after (2) pairing. b Summary of the results. c The t-LTP requires mGluR5. The EPSP slopes monitored in control slices (black triangles, n = 9) and in slices treated with the mGluR antagonist LY341495 (100 µm, red squares, n = 6), the group I mGluR antagonist LY367385 (100 µm, gray triangles, n = 6) or the mGluR5 antagonist MPEP (500 µm, blue triangles, n = 6) are shown following post-before-pre pairing. The traces show the EPSP before (1) and 30 min after (2) pairing. d Summary of the results. e The t-LTP requires activation of presynaptic mGluR5s. Time course of t-LTP induction in control conditions (black triangles, n = 9) and with the postsynaptic neuron (red triangles, n = 6) or astrocytes (gray triangles, n = 10) loaded with GDPβS (1 mm). Inset: Traces show the EPSP before (1) and 30 min after pairing (2) in control slices or when the postsynaptic neuron or astrocytes are loaded with GDPβS. f Summary of the results. **p < 0.01, ***p < 0.001, one-way ANOVA + Holm–Sidak. Error bars represent the S.E.M. Source data are provided as a Source Data file.
