Fig. 4: t-LTP requires postsynaptic calcium and NO.
From: Astrocyte-mediated switch in spike timing-dependent plasticity during hippocampal development
a t-LTP is prevented by loading BAPTA (20 mm) into the postsynaptic recording pipette. Nimodipine (10 µm), thapsigargin (10 µm) and ryanodine (100 µm) loading into the postsynaptic neuron via the patch pipette prevented t-LTP induction. The EPSP slopes shown were monitored in control slices (black triangles, n = 11) and in slices treated with BAPTA (red squares, n = 6), nimodipine (gray squares, n = 6), thapsigargin (blue squares, n = 6) and ryanodine (pink squares, n = 6). The traces show the EPSP before (1) and 30 min after (2) pairing. b Summary of the results. c Time course of the effect of post-before-pre pairing in control conditions (black triangles, n = 6) and with cPTIO (100 μm, red squares, n = 6) and L-NAME (100 µm, gray squares, n = 6) loaded into the postsynaptic neuron. Insets: the traces show the EPSPs before (1) and 30 min after pairing (2). d Summary of the results. ***p < 0.001, one-way ANOVA + Holm–Sidak. The error bars represent the S.E.M. Source data are provided as a Source Data file.
