Fig. 1: Transient absorption data in the visible collected over 12 decades in time.
From: Confinement in crystal lattice alters entire photocycle pathway of the Photoactive Yellow Protein
A selection of transient absorption spectra as a function of time delay are shown, upon excitation with a 50-fs laser pulse centered at 475 nm, for a PYPS and b PYPC. The temporal evolution is indicated by the black arrows. In PYPC, a scattered-light artifact is visible at the wavelength of excitation. c Wavelength traces at 404, 444 and 494 nm of PYPS (black) and PYPC (red), see Supplementary Figs. 1 and 2 for traces at additional wavelengths. The data from the current study with sub-picosecond time resolution has been merged with data taken from Yeremenko et al.19 with microsecond time resolution, with the latest time point at 48 ms (crystal) or 1 s (solution). Wavelength is indicated in the ordinate label. Note that the time axis is linear until 1 ps (after the maximum of the instrument response function (IRF)), and logarithmic thereafter. A single scaling parameter has been used to connect the data from the experiments with sub-picosecond and microsecond time resolution. PYPS was measured at pH 8, PYPC at pH 6.5. PYPS time traces collected at pH 6 are shown in Supplementary Fig. 1 and exhibit corresponding dynamics.
