Fig. 5: UV–Vis SADS of PYP.
From: Confinement in crystal lattice alters entire photocycle pathway of the Photoactive Yellow Protein
The SADS in solution (a) and crystalline (b) form have been resolved from fitting the target model shown in Fig. 4 to the experimental data, and display the bleached ground state, and stimulated emission, as negative features and induced absorption as positive features. The characteristic blue (≈370 nm) absorption of pCA in the pB states signals protonation of pCa. The region of the ground state bleach in the PYPC dataset appears to be diminished with respect to that in PYPS; this is an effect of the high absorption of the crystals around 444 nm. Note that data from Yeremenko et al.19 has been added to the analysis to more reliably estimate the dynamics and SADS on the ~10–300 ms timescale and to span the full photocycle dynamics up to 1 s. Source data are provided as a Source Data file.
