Fig. 7: Distinct functions of FANCJ and DDX11 in response to G4-stabilization. | Nature Communications

Fig. 7: Distinct functions of FANCJ and DDX11 in response to G4-stabilization.

From: Warsaw Breakage Syndrome associated DDX11 helicase resolves G-quadruplex structures to support sister chromatid cohesion

Fig. 7

a Alignment of sequences of FANCJKO and DDX11KO-FANCJKO at the crFANCJ target site. b Cell growth relative to RPE1-TP53KO cells, as assessed by counting three days after seeding. Bars represent mean of two biological replicates. c Clonogenic survival assay was performed in cells treated as indicated for 24 h, and subsequently cultured for nine days. Representative images from two separate experiments are shown. d Cell titer blue assessed viability following five days of the indicated treatments. Lines indicate the mean of three technical replicates. e, f Counts of γH2AX (e) and TP53BP1 (f) foci per cell after 24 h treatments (n = 50 cells for two separate experiments). Boxes extend from the 25th to the 75th percentile, whiskers from the smallest to the largest value, lines indicate the median.

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