Fig. 5: Rcr3 variants are diverging on Avr2 inhibition and HR signalling.
From: Evolution of a guarded decoy protease and its receptor in solanaceous plants
a Activity profiling of Rcr3 homologs. Rcr3 homologs were co-expressed with the silencing inhibitor P19 in N. benthamiana leaves by agroinfiltration. Apoplastic fluids were isolated at 2 dpi, preincubated for 45 min with or without 100 µM E-64, 4 µM Avr2 or Avr2Δ6, or DMSO, and labelled for 3 h with 2 µM MV201. Samples were separated on SDS-PAGE gels and scanned for fluorescence. b Tomato and potato Rcr3 homologs can trigger a HR, while the pepper, eggplant, Nicotiana and Petunia Rcr3 homologs do not trigger responses. Rcr3 homologs were co-expressed with Avr2 and Cf-2 by mixing Agrobacterium cultures (OD600 = 0.25 each) in a 1:1:1 ratio. Pictures were taken at 8 dpi.
