Fig. 2: Characterization by in-gel fluorescence and in solution. | Nature Communications

Fig. 2: Characterization by in-gel fluorescence and in solution.

From: A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells

Fig. 2: Characterization by in-gel fluorescence and in solution.

a In-gel detection of the quenched SNAP-tag substrate of 600 μM SNAPSwitch or fluorescent SNAP-Cell 647-SiR with or without 5 μM SNAP-tag protein incubated at 37 °C for 30 min. b Densitometric analysis of SNAPSwitch activation with and without SNAP-tag incubation. c Fluorescence activation of 0.10 μM SNAPSwitch in the presence of SNAP-tag protein at 27 °C over 1.5 h. The mean of three wells is plotted with error bars representing the standard deviation (n = 3). The data are fitted with an exponential one-phase association curve.

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