Fig. 6: Uptake, trafficking and delivery of oligonucleotide.
From: A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells
HEK293 cells were transfected to transiently express H2A-mTurquoise-SNAP-tag and analysed by flow cytometry. a SNAP-Cell SiR 647 signal in cells gated positive or negative for mTurquoise expression. b Association of Lipofectamine 3000 complexes with cells transfected with H2A-SNAP or an empty plasmid. c SNAPSwitch signal in cells positive for mTurquoise expression with the signal from negative cells subtracted as background. Deconvolved fluorescence microscopy images of oligonucleotide labelled with SNAPSwitch and incubated with HEK293 cells for 16 h, transfected with d Cyto-SNAP, e H2A-SNAP and f an empty plasmid and surface stained with wheat germ agglutinin Alexa Fluor 488, scale bar = 10 μm. g HEK cells stably expressing TfR-SNAP, Cyto-SNAP or H2A-SNAP and transfected with Lipofectamine 3000 complexes containing AF488 and SNAPSwitch oligonucleotides over time, analysed by flow cytometry. The mean fluorescence intensity (a–c) or ratio (g) is plotted with error bars representing the standard deviation, from two experiments in duplicate (a–c, n = 4) or one experiment in triplicate (g, n = 3). Source data are presented in a Source Data file.
