Fig. 5: P granule components are targeted by small RNAs and mechanistic model of DEPS-1 function in small RNA pathways. | Nature Communications

Fig. 5: P granule components are targeted by small RNAs and mechanistic model of DEPS-1 function in small RNA pathways.

From: DEPS-1 is required for piRNA-dependent silencing and PIWI condensate organisation in Caenorhabditis elegans

Fig. 5: P granule components are targeted by small RNAs and mechanistic model of DEPS-1 function in small RNA pathways.The alt text for this image may have been generated using AI.

a 55 P granule factors are targeted by endo-siRNAs. b The 22Gs of 10P granule factors are differentially regulated in deps-1(bn121) mutant. c Wild-type DEPS-1 binds directly to the PIWI domain of PRG-1 via the PBS motif in the N-terminal. The PRG-1 and DEPS-1 clusters interact to form elongated perinuclear condensates. Intact PRG-1/DEPS-1 complex maintains normal morphology of PRG-1 and MUT-16 condensates. Efficient gene silencing mediated by the piRNA pathway ensues (top panel). DEPS-1 PBS mutant cannot associate with PRG-1 in perinuclear condensates and becomes dispersed in the cytoplasm. This results in an increase in the intensity of PRG-1 and MUT-16 condensates. Additionally, the steady-state level of secondary endo-siRNA from multiple pathways is reduced in the presence DEPS-1 PBS mutant. In particular, the piRNA pathway cannot mediate gene silencing (bottom panel). This figure was created by Claudia Flandoli.

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