Fig. 1: An auditory association training paradigm.
From: Single-neuron representation of learned complex sounds in the auditory cortex
a Cartoon illustration of the sound-water association training paradigm and experiment setup. 2PM: two-photon microscope; E-phys: single-cell loose-patch pipette. b Learning curve, animals trained and tested with broadband noise (BBN), n = 6 mice. Error bars: +/− s.e.m., same for all the other panels in Fig. 1. c Spontaneous licking analysis. n.s., P > 0.05. n = 6 mice. d Psychometric test in animals trained with BBN and tested with a range of pure tones; P = 3.30e−3, P = 3.30e−3, P = 3.30e−3, P = 5.50e−3, P = 1.10e−3, P = 3.30e−3, P = 2.20e−3, P = 3.30e−3, P = 5.50e−3, P = 3.30e−3, P = 4.40e−3, BBN versus different pure tones, respectively, two-sided paired bootstrap test, Bonferroni corrected, n = 6 mice. e Cartoon illustration of the 2-tone training paradigm. f Learning curve, animals trained with 2 tones (2.0 kHz or 12.1 kHz), n = 6 mice. g Psychometric test, 2-tone-trained animals, ΔFreq (octaves): frequency difference between the test tone and one of the two trained tones (to the closer one); P = 4.20e−3, P = 3.00e−3, P = 6.00e−4, P = 3.00e−3, P = 7.80e−3, P = 9.00e−3, two-sided unpaired bootstrap test, the test tone versus other tones, respectively, Bonferroni corrected, n = 6 mice. h Cartoon illustration of the 2-chord training paradigm. i Learning curve, animals trained with 2 chords; n = 6 mice. j Psychometric test, 2-tone-trained animals tested with the two trained chords and each of their constituent tones; P = 0.0128, P = 6.00e−3, P = 5.20e−3, P = 3.20e−3, chord 1 versus its constituent tones, respectively, two-sided paired bootstrap test, Bonferroni corrected, n = 6 mice; P = 5.60e−3, P = 1.20e−3, P = 1.20e−3, P = 8.00e−4, chord 2 versus its constituent tones, respectively, two-sided paired bootstrap test, Bonferroni corrected, n = 6 mice. In psychometric testing sessions, different sounds were played in randomized trial orders, and behavioural responses until a total consumption of 20 droplets in one session were used for analysis. Data with error bars are presented as the mean ± s.e.m. **P < 0.01, *P < 0.05.
