Fig. 1: Mitochondrial DNA nucleoids are transported via MDT. | Nature Communications

Fig. 1: Mitochondrial DNA nucleoids are transported via MDT.

From: ER-mitochondria contacts promote mtDNA nucleoids active transportation via mitochondrial dynamic tubulation

Fig. 1

a Representative time-lapse images of MDT in a Cos-7 cell expressing Tom20-GFP and TFAM-mCherry demonstrate the transportation of TFAM-labeled nucleoids spatially linked to the MDT process. White arrowheads mark the tips of tubules generated by the MDT processes. Orange arrowheads indicate the sites of nucleoids. Additional examples are shown in Supplementary Fig. 2a–c. b Percentage of nucleoids near the MDT initiation sites that get transported into the thin tubule (blue box) or remain at the MDT initiation sites (orange box) for over 2 min, n = 42 events. c The trajectory and velocity of the nucleoid and that of the dynamic tubule during the tubulation process in a. d Spatial cross-correlation analysis of the tubulation process (Tom20-GFP) and nucleoid (TFAM-mCherry) trajectories, n = 17 events examined over three independent experiments. Data are shown as mean ± SEM. e Percentage of nucleoids in dynamic tubules transported to the tip of the tubules (orange box) or not the tip of the tubules (blue box) during the MDT process, n = 17 events. f Representative time-lapse images of MDT in a Cos-7 cell expressing Tom20-GFP and TFAM-mCherry demonstrate unsynchronized motility of TFAM-labeled nucleoids and the MDT process. White arrowheads mark the tips of tubules generated by the MDT processes. Orange arrowheads indicate the sites of nucleoids. g The trajectory and velocity of the mitochondrial DNA nucleoid and that of the dynamic tubule during the tubulation process in f. Scale bars: a, 1 μm and f, 2 μm. Source data are provided as a Source data file.

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