Fig. 4: Acinar metaplastic cells are heterogeneous.

In the middle of the figure a snapshot of Fig. 2b is presented, showing tdTomato-positive and stomach cluster cells. a Dot plot of selected differentially expressed genes in early (control, 17 days and 6 weeks PTI samples) and late (3, 5, and 9 months PTI samples), acinar, ductal and metaplastic clusters of cells as indicated on the y-axis. Dot size represents the expression level and color represents the percentage of cells that express the indicated gene, in each cluster. b Histological sections of mouse pancreas 4 months PTI, immunostaining with anti-Ins1. A green arrow indicates an islet; a black arrow indicates a stained cell that is localized to PanIN. Scale bar 100 µm. c Quantities analysis of metaplastic cell-types and states in PanIN lesions and in ductal structures. Data collected from slices from three different mice for each antibody (n = 3), and is based on the counting of 24,808 cells, see Supplementary Data 9. The bars represent the mean values and ±SD. d, e, h Metaplastic tuft-like cells. Immunostaining using anti-Dclk1 antibody, d control, 5 months PT mouse, e 5 months PRT mouse, scale bars 100 µm. h Violin plot showing the expression of Dclk1 and Pou2f3. f, g, i Senescent cells. Immunostaining using anti-Tgfb1, f control, 5 months PT mouse, g 5 months PRT mouse, scale bars 100 µm. i Violin plot showing the expression of Gjb4, Tgfb1, and Cdkn2a. j Metaplastic chief-like cells, violin plot showing the expression of Muc6, Pgc. m, n, k Metaplastic stomach pit-like cells. Immunostaining using anti-Tff1 antibody, m control, 5 months PT mouse, n 5 months PRT mouse, scale bars 100 µm. k Violin plot showing the expression of Gkn1, Tff1, and Muc5ac. o, p, l Metaplastic cycling cells. Immunostaining with anti-Mki67 antibody, o control, 5 months PT mouse, p 5 months PRT mouse, scale bars 100 µm. l Violin plot showing the expression of Mki67.