Fig. 1: FIB milling and tomograms.
From: Assembly intermediates of orthoreovirus captured in the cell
a SEM image of vitrified infected cells on EM grid prior to FIB milling. Scale bar 1 mm. b SEM image after FIB milling. White arrow shows one of the four-milled lamellae. Scale bar 100 μm. c, d Example tomogram of particles within cell (d shows a close up view). Scale bars 3000 and 1800 Å, respectively. e–g Representatives of stars, empty, and full virion-like particles, respectively taken from SIRT reconstruction by Tomo3D44. All three images are at the same scale and the scale bar corresponds to 450 Å. These images are representative of three lamella from which eight tomographic series were collected.
