Fig. 3: Butyrate promotes IL-22 production through GPR41 and HDAC inhibition. | Nature Communications

Fig. 3: Butyrate promotes IL-22 production through GPR41 and HDAC inhibition.

From: Intestinal microbiota-derived short-chain fatty acids regulation of immune cell IL-22 production and gut immunity

Fig. 3: Butyrate promotes IL-22 production through GPR41 and HDAC inhibition.

a, b CBir1 Tg CD4+ T cells were cultured with APCs and Cbir1 peptide with or without butyrate (0.5 mM) ± AR420626 (5 µM) or/and TSA (10 mM) under Th1 conditions (n = 3/group). IL-22 mRNA (a) and protein (b) were measured by qRT-PCR and ELISA at 60 h. IL-22 production was measured by flow cytometry on day 5 (c). d CD4+ T cells were cultured with anti-CD3/CD28 mAbs under Th1 conditions with or without butyrate (0.5 mM) or TSA (10 mM) (n = 3/group). Cells were collected at 24 h for analysis of HDAC activity at fluorescence intensity at excitation/emission (490/525 nm) by using the HDAC Activity Assay Kit. One representative of three independent experiments was shown. Data were expressed as mean ± SD. Statistical significance was tested by two-tailed one-way ANOVA. a ****p < 0.0001, **p = 0.0016, *p = 0.0282; b ****p < 0.0001; c ****p < 0.0001, ***p = 0.0002, **p = 0.0054; d *p = 0.0144, ***p = 0.0004.

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