Fig. 6: Butyrate promotes HIF1α binding to Il22 promoter in CD4+ T cells. | Nature Communications

Fig. 6: Butyrate promotes HIF1α binding to Il22 promoter in CD4+ T cells.

From: Intestinal microbiota-derived short-chain fatty acids regulation of immune cell IL-22 production and gut immunity

Fig. 6: Butyrate promotes HIF1α binding to Il22 promoter in CD4+ T cells.

a Schematic diagram of HIF1α binding to Il22 promoter. b WT CD4+ T cells were activated with anti-CD3/CD28 mAbs under Th1 conditions for 2 days (n = 3/group). HIF1α binding to Il22 promoter was analyzed by CHIP assay. c–e WT CD4+ T cells were cultured under Th1 conditions with or without butyrate (0.5 mM) for 2 days (n = 3/group). HIF1α binding to Il22 promoter was analyzed by CHIP assay (c). The H3K9 acetylation (d) and trimethylation (e) levels in HIF1α-binding site on Il22 promoter were assessed by CHIP assay. One representative of three independent experiments (b, c), or two independent experiments (d, e) was shown. Data were expressed as mean ± SD. Statistical significance was tested by two-tailed unpaired Student t-test. b **p = 0.0047; c *p = 0.0278; d *p = 0.0105; e **p = 0.0094.

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