Fig. 7: Biological network analysis of genes associated with macrophage clusters.
From: Ontogeny of arterial macrophages defines their functions in homeostasis and inflammation
GO term and pathway analysis of EMP- and BM-derived macrophages in response to AngII. a Dot plot showing the top 15 genes, which are differentially expressed between EMP- and BM-derived macrophages (upper panel). Functionally grouped networks (lower panels) depicting biological processes and pathways associated with genes that are significantly upregulated in Yfp+ or Yfp− macrophages (p value < 0.05, Log2FC > 0.25). Each node represents a specific biological process or pathway, and edges indicate the relations between different terms based on their kappa score level (>0.4). Nodes of the same color belong to functionally similar terms or pathways. The node size represents the term enrichment significance (P value < 0.0005 to <0.05). b Networks of functionally grouped pathways and processes associated with secretory markers of Yfp+ or Yfp− macrophages. Genes with capability of producing secretory proteins were shortlisted from all significantly upregulated genes in each population and subjected to pathway enrichment analysis. The marker genes are identified using Seurat FindMarker function based on Wilcoxon Rank Sum test. ClueGo performs the enrichment test based on the hypergeometric distribution and Bonferroni correction.
