Fig. 5: Gene expression in cycling blood-borne cells in steady state and during WBR. | Nature Communications

Fig. 5: Gene expression in cycling blood-borne cells in steady state and during WBR.

From: Integrin-alpha-6+ Candidate stem cells are responsible for whole body regeneration in the invertebrate chordate Botrylloides diegensis

Fig. 5

a Isolation of cycling G2/M cells by flow cytometry. Cells were stained with the live DNA stain Dye Cycle Ruby. Linear analysis of fluorescence intensity plotted against forward scatter (cell size) shows a clear separation of G2/M cells with 4n DNA content and G0/G1 cells with 2n DNA content. G2/M cells comprise 10% of live cells. b qPCR analysis showing expression of, integrin-alpha-6, cyclin b, pou3, notch1, notch2, hes1, frizzled 5/8, disheveled (dshv), and beta catenin in G2/M cells in steady state (0 h). Data are expressed as fold changes normalized to G0 cells from three independent experiments. Box plot shows interquartile range (the distance between the upper and lower quartiles) and whiskers indicate the minimum and maximum of the data. c qPCR analysis showing expression of cyclin b, integrin-alpha-6, pou3, notch1, notch2, and frizzled 5/8 in G2/M cells at 24 and 48 h post injury. Data are expressed as fold changes normalized to actin, from three independent experiments. Box plot shows interquartile range (the distance between the upper and lower quartiles) and whiskers indicate the minimum and maximum of the data. Source data are provided as a Source Data file.

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