Fig. 5: Effects of deletions of putative enhancers in the Tspan32 gene locus in differentiating MEL cells. | Nature Communications

Fig. 5: Effects of deletions of putative enhancers in the Tspan32 gene locus in differentiating MEL cells.

From: Hyperacetylated chromatin domains mark cell type-specific genes and suggest distinct modes of enhancer function

Fig. 5

a Normalized gene expression for Tspan32, Cd81 and Tssc4 measured by qrt-PCR in wt (MEL) and upon deletion of the indicated enhancer regions (0.7, −10, −17). P-values were calculated using a two-tailed Student’s t-test; *P < 0.05, **P < 0.005, ***P < 0.0005. b, c Bar graphs showing % of input control for the indicated probes derived from qrt-PCR analysis of ChIPs using antibodies specific for H3K4Me2 (b) or H3K27Ac (c). Amylase indicates a control probe within the inactive amylase gene locus. Results of all panels are means + s.e.m of at least three independent experiments. Each colored circle represents the average of three technical replicates of an independent single-cell-derived homozygous enhancer knockout clone.

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