Fig. 2: AR expression and nuclear translocation in THP-1 cells.

a Expression of the macrophage marker CD68 (green) in THP-1 cells at steady state (no treatment) and following 2 days of PMA stimulation. Nuclei were stained with DAPI (dark blue). Scale bar = 50 µm. Representative of three images per condition. b RT-PCR showing AR expression at the RNA level in human cancer cell lines of prostate epithelial (CWR-R1) and monocytic (THP-1^PMA;IFNG;LPS) origin. GAPDH was used as a house-keeping control gene. This experiment was performed two times. c Western blot showing AR expression at the protein level in human cell lines originated from prostate cancer (LNCaP), melanoma (M14) and monocytic leukaemia (THP-1^PMA;IFNG;LPS). β-Actin was used as a loading control. This experiment was performed two times. Source data are provided as a source datafile. d Western blot showing AR expression at the protein level in the subcellular chromatin fraction of THP-1^PMA;IFNG;LPS cells and CWR-R1 human PCa cells upon R1881 stimulation. Pol-II was used as a loading control of the chromatin fraction. This experiment was performed two times. Source data are provided as a source datafile.