Fig. 4: AR signalling in THP-1 cells affects prostate tumour growth in vivo.

a Representative images of both normal chick embryo chorioallantoic membrane (CAM) and PC3 PCa cells derived tumours growing on the CAMs replenished with CM of DMSO or RD162-treated THP-1^PMA;IFNG;LPS cells, CCL2 or NaCl,. The only tumour grown in NaCl-treated CAM conditions is shown. All other NaCl-treated CAMs showed no sign of tumour growth. Scale bar = 500 µm. b Growth curves of PC3 tumours grafted into CAMs showing tumour volume over time in the different treatment conditions. Datapoints represent the average tumour volume as a percentage of the tumour volume at the start of treatment (EDD10). Error bars represent s.e.m. of 6–10 biological replicates per condition in one experiment with one batch of THP-1^PMA;IFNG;LPS cell CM (DMSO CM and RD162 CM). *: p = 0.02. p-value comparing DMSO CM versus RD162 CM was calculated using a Two-way Anova with a cutoff for significance of 0.05. Source data are provided as a source datafile. c The expression of human vimentin in disseminated PC3 cells into CAM tissue distant from the primary tumour site (normal CAM) in the different treatment conditions and normalized to the reference gene human Cyclophylin A (2^−dCt). Error bars represent the s.e.m. of 5–9 biological replicates per condition in one experiment with one batch of THP-1^PMA;IFNG;LPS cells CM (DMSO CM and RD162 CM). p = 1.0. p-value comparing DMSO CM versus RD162 CM was calculated using a One-way Anova test with a cutoff for significance of 0.05. NS: no significant difference. Source data are provided as a source datafile.