Fig. 6: AR regulates expression of TREM-1 associated genes in THP-1 cells.

a RT-QPCR analysis to assess fold change expression of TREM-1 (2^-ΔΔCt) in THP-1^PMA;IFNG;LPS cells upon R1881 stimulation compared to vehicle control (set at 1) and normalized to TBP expression. Datapoints show the mean value of three technical replicates in each experiment, while error bars show the s.e.m. of two independent experiments. Source data are provided as a source datafile. b RT-QPCR analysis to assess the fold change expression (2^−ΔΔCt) upon R1881 exposure of 28 TREM-1-associated genes in THP-1^PMA;IFNG;LPS cells, relative to TBP expression and normalized to vehicle conditions (set at 1). Each datapoint represents the mean relative gene expression in three technical replicates. The mean fold change expression (vehicle over R1881) of all genes is indicated, while error bar show the s.e.m. of two independent experiments. *: p = 0.001. p-Value for the comparison of fold change of all genes separately upon vehicle exposure (set at 1) versus R1881 exposure, calculated using an unpaired parametric Studentʼs t test with a cutoff for significance of 0.05. Source data are provided as a source datafile. c RT-QPCR analysis to assess fold change expression (2^−ΔΔCt) upon R1881 exposure of 5 TREM-1 associated chemokines in THP-1^PMA;IFNG;LPS cells, relative to TBP expression. Green bars show R1881 conditions normalized to vehicle (red box and dotted line). Datapoints show mean values and error bars the s.e.m. of two independent experiments with three technical replicates each. Source data are provided as a source datafile. d Fold change expression (2^−ΔΔCt) of TREM-1 associated chemokines in THP-1^PMA;IFNG;LPS cells normalized to TBP expression. Cells were exposed to scramble peptide or to the inhibitory TREM-1 peptide (iTREM1) in combination with R1881 and/or RD162. Values were normalized to scramble peptide alone. Datapoints show mean values and error bars the s.e.m. of two independent experiments with three technical replicates each. Source data are provided as a source datafile. e Kaplan–Meier curves of disease/progression-free survival in PCa patients in relation to levels of TREM-1 expression (TCGA database, No. of patients = 491). Z-score of ± 1. Fifty-seven cases with a high TREM-1 expression (red line; 18 relapses/disease progressions) and 434 cases with a low TREM-1 expression (blue line; 73 relapses/disease progressions). P = 0.0042. Log-rank chi square test was used to calculate the p-value with a cutoff for significance of 0.05. Source data are provided as a source datafile.