Fig. 7: TREM-1 signalling in THP-1 cells promotes PCa migration and invasion. | Nature Communications

Fig. 7: TREM-1 signalling in THP-1 cells promotes PCa migration and invasion.

From: Androgen receptor signalling in macrophages promotes TREM-1-mediated prostate cancer cell line migration and invasion

Fig. 7

a Representative images of three independent scratch assays of CWR-R1 cells cultured in CM of THP-1PMA;IFNG;LPS cells stimulated with vehicle, RD162, scramble peptide or inhibitory TREM-1 peptide (iTREM-1). Three technical replicates were included in each experiment. Scale bar = 200 µm. b Quantification of three independent migration scratch assays. Closure of the scratch by CWR-R1 cells after 24 h relative to day 0. Datapoints show mean values of three technical replicates in each experiment. Error bars represent the s.e.m. *: p = 0.05, one-way Anova test was used to calculate the p-value with a cutoff for significance of 0.05. Source data are provided as a source datafile. c Representative images of two transwell invasion assays (three technical replicates) of CWR-R1 cells cultured in normal medium alone or in a combination with CM of THP-1PMA;IFNG;LPS cells stimulated with vehicle, RD162, vehicle CM with scramble peptide or vehicle CM with TREM-1 inhibitory peptide. Cells that invade the matrigel and passed through the membrane after 72 h of culture were stained with crystal violet. d Quantification of the transwell invasion assays. Invasion was quantified by optical density (OD) of crystal violet stained cells. Datapoints show mean values and error bars the s.e.m. of two independent experiments with three technical replicates each. Source data are provided as a source datafile. e Representative images of two transwell migration assays (three technical replicates) of CWR-R1 cells cultured in normal medium alone or in combination with CM of THP-1PMA;IFNG;LPS cells stimulated with vehicle or RD162. Additionally, CWR-R1 cells cultured in normal medium in combination with CM of vehicle stimulated THP-1PMA;IFNG;LPS cells alone or supplemented with blocking antibodies against CCL13, CCL2, CCL3, CCL7, CXCL8 or the combination of all. Cells that passed through the membrane after 72 h of culture were stained with crystal violet. f Quantification of the migration transwell assays. Invasion was quantified by optical density (OD) of crystal violet stained cells. Datapoints show mean values and error bars the s.e.m. of two independent experiments with three technical replicates each. Source data are provided as a source datafile.

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