Fig. 8: AR signalling upregulates tissue macrophage-related markers in THP-1 cells.

a Snapshot of AR and H3K27ac sites proximal to genes associated with macrophage differentiation in THP-1^PMA;IFNG;LPS cells (IL-10, CD209, CD206 and CD163). Genomic coordinates, gene name and tag counts are indicated. AR peaks in vehicle and R1881 conditions are depicted in blue and red, respectively. H3K27ac peaks in vehicle and R1881 conditions are depicted in green and purple, respectively. Range of normalized read counts is shown on the y axis. b RT-QPCR analysis to assess fold change expression (2^−ΔΔCt) of CD163, IL-10, CD206, CD209 in THP-1^PMA;IFNG;LPS cells upon R1881 stimulation, relative to GAPDH expression and normalized to vehicle conditions (dotted line). Datapoints show mean values and error bars the s.e.m. of two independent experiments with three technical replicates each. Source data are provided as a source datafile. c Flow-cytometry analysis of surface markers CD206 and CD163 expression in MDM cells. Unstained cells (upper-left) were used as negative control. Twenty-four hrs vehicle stimulated (upper-right), R1881 (10⁻¹⁰ M) stimulated (lower-left) and simultaneous R1881 (10⁻¹⁰ M) and RD162 (10⁻⁷ M) stimulated cells (lower-right). The percentage of double positive CD163+/CD206+THP-1^PMA;IFNG;LPS cells in the various conditions is represented in bold. Plots are representative for three independent experiments.