Fig. 1: Design of Engineered Genetic Incompatibility (EGI).
From: Engineering multiple species-like genetic incompatibilities in insects
a Schematic diagram of required genotypes for EGI. L, dominant lethal gene; l, wild-type allele (null); S, dominant susceptible allele; s, recessive resistant allele. b X-ray crystal structure of S. pyogenes Cas9 (PDB ID: 6o0z, left) and diagram of dominant lethal gene product, dCas9-VPR. c Interaction of dCas9-VPR with resistant (top) or susceptible (bottom) alleles. Blue square represents a mutation that abrogates dCas9 binding. RNAP, RNA polymerase.
