Fig. 1: Identification of SARS-CoV-2 RBD binding sdAbs. | Nature Communications

Fig. 1: Identification of SARS-CoV-2 RBD binding sdAbs.

From: Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain

Fig. 1: Identification of SARS-CoV-2 RBD binding sdAbs.The alternative text for this image may have been generated using AI.

a The purified recombinant proteins of SARS-CoV-2 RBD and sdAbs were separated by SDS-PAGE and stained with Coomassie Blue. Lanes: 1, SARS-CoV-2 RBD; 2, 1E2; 3, 2F2; 4, 3F11; 5, 4D8; 6, 5F8. b–f Five sdAbs binding to SARS-CoV-2 RBD measured by SPR. Two-fold serial dilutions from 25 nM sdAb injected onto the captured RBD protein. Kinetic data from one representative experiment were fit to a 1:1 binding model. The profiles are shown for 1E2 (b), 2F2 (c), 3F11 (d), 4D8 (e), and 5F8 (f). g Kinetics of binding between SARS-CoV RBD and sdAb 5F8. h Summary of SPR kinetic and affinity measurements. The equilibrium dissociation constant (KD), the association constant (kon) and the dissociation constant (koff) are presented. N.D. means not detected.

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