Fig. 5: Direct culture of RMS patient cells for drug profiling and therapy guidance. | Nature Communications

Fig. 5: Direct culture of RMS patient cells for drug profiling and therapy guidance.

From: Phenotypic profiling with a living biobank of primary rhabdomyosarcoma unravels disease heterogeneity and AKT sensitivity

Fig. 5

a Scheme depicting the clinical course of patient RMS-ZH002. b Bright-field image of cells isolated from the lymph node biopsy of patient RMS-ZH002. Scale bar 400 μm. The displayed image is representative of n = 1 individual cell isolation. c Fluorescence in situ hybridization detection of the FOXO1 gene using probes binding to genomic sites N- (red) and C-terminal (green) of the gene. The displayed image is representative of n = 1 fluorescence in situ hybridization experiment. d Heat map depicting the activity of indicated agents used at a final concentration of 500 nM on cells isolated from PDX RMS-ZH002 and on cells isolated from the lymph node biopsy. Each color in the heat map indicates the percentage of remaining cell viability after treatment relative to vehicle control: 0–30% (red), 30–60% (orange), 60–79% (light blue) and >79% (white). e, left panel Heat map depicting effect of panobinostat and bortezomib alone and in combinations on viability of RMS-ZH002 patient cells isolated from the lymph node biopsy (Mean; n = 2 biological replicates). Clinically applicable concentrations of the two drugs are framed with a green box. Right panel, Heat map depicting synergism between panobinostat and bortezomib using zero interaction potency model analysis. Source data are provided as source data file.

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