Fig. 6: Manipulation of MT polymerisation dynamics in subcellular ROIs of rat primary hippocampal neurons using AzTax3MP. | Nature Communications

Fig. 6: Manipulation of MT polymerisation dynamics in subcellular ROIs of rat primary hippocampal neurons using AzTax3MP.

From: Photoswitchable paclitaxel-based microtubule stabilisers allow optical control over the microtubule cytoskeleton

Fig. 6: Manipulation of MT polymerisation dynamics in subcellular ROIs of rat primary hippocampal neurons using AzTax3MP.The alternative text for this image may have been generated using AI.

Data related to Supplementary Movies 5–8. a, b Cultured primary neurons (9 days in vitro) transfected with EB3-tdTomato treated with 1% DMSO were initially imaged for EB3 for 10 min while a ROI (violet box) was pulsed with 405 nm light, establishing baselines for EB3 activity in the cell and in the ROI, which were demonstrated to be light-independent. The same neurons were then exposed to 0.5 µM AzTax3MP and immediately imaged for another 10 min; during this time the same ROI (violet box) was pulsed with 405 nm light beginning at 2 min into the acquisition (indicated by dotted lines). a Cell images with areas marked, and corresponding kymographs of these areas. The ROI pulsed with 405 nm is boxed in violet, the non-ROI areas (not pulsed with 405 nm) are boxed in orange and green. Scale bars indicate 10 µm. b Normalised area-average pixel EB3 intensities (with s.e.m.), for areas treated with or without AzTax3MP and 405 nm pulsing (n  =  4 cells). For clarity, data for cosolvent-only areas are shown as spline fits; see Supplementary Note 4 for further details.

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