Fig. 5: eIF2D and DENR modulate stress response phenotypes.

a Survival rate of second instar larvae of the indicated genotypes when fed with normal food (white bars) or amino acid deprived food (4% sucrose, red bars) for 8 h. Data represent the mean from five independent experiments with 20 animals in each trial, and error bars represent standard error. b qPCR analysis of 4E-BP mRNA levels in larvae from a. Data are the mean from three independent experiments, with error bars representing standard error. In both a, b, p values were calculated using the two-tailed t-test with **p < 0.001, ***p < 0.0001 and n.s. not significant. c Photoreceptor degeneration in the ninaE^G69D/+ adRP model in control and perk mutants as assessed by Rh1-GFP fluorescence that allows for visualization of photoreceptors in adult pseudopupils. Note that the lines for w¹¹¹⁸ (solid black) and perk^e01744 (solid red) overlap. The difference in the course of retinal degeneration between the following pairs is statistically significant as assessed by the Log-rank (Mantel-Cox) test (p < 0.0001): w¹¹¹⁸ and w¹¹¹⁸;;ninaE^G69D/+, perk^e01744 and perk^e01744,ninaE^G69D/+, w¹¹¹⁸;;ninaE^G69D/+ and perk^e01744,ninaE^G69D/+. (n = 100). d Photoreceptor degeneration with ninaE^G69D/+ monitored in various eIF2D mutant backgrounds. Note that the curves for the control Df.eIF2D^WT (solid black), Df.ΔeIF2D (solid red) and Df.eIF2D^D109A (solid blue) overlap for the early time points. The difference in the course of retinal degeneration between the following pairs is statistically significant as assessed by Log-rank (Mantel-Cox) test (p < 0.0001): Df.eIF2D^WT and Df.eIF2D^WT,ninaE^G69D/+, Df.ΔeIF2D and Df.ΔeIF2D,ninaE^G69D/+, Df.ΔeIF2D and Df.ΔeIF2D,ninaE^G69D/+, Df.eIF2D^WT,ninaE^G69D/+ and Df.ΔeIF2D,,ninaE^G69D/+, Df.eIF2D^WT,ninaE^G69D/+ and Df.eIF2D^D109A,ninaE^G69D/+. (n = 100). Please see Source Data Files for the raw data in a–d.