Fig. 6: eIF2D and DENR regulate the translation of the ATF4 ORF. | Nature Communications

Fig. 6: eIF2D and DENR regulate the translation of the ATF4 ORF.

From: Translational induction of ATF4 during integrated stress response requires noncanonical initiation factors eIF2D and DENR

Fig. 6: eIF2D and DENR regulate the translation of the ATF4 ORF.The alternative text for this image may have been generated using AI.

a–i Expression of the ATF4 5′UTR-dsRed reporter in fat bodies of control (Df.eIF2DWT), eIF2D transheterozygous null (Df.ΔeIF2D) and point (Df.eIF2DD109A) mutants. The reporter utilizes a dsRed reporter bearing a nuclear localization sequence. Control GFP expression is driven by Dcg-GAL4. Larvae in b, e, h were subjected to 4 h of amino acid deprivation and those in c, f, i were fed Tunicamycin for 4 h. Data are representative images collected from two independent biological experiments with ten animals in each trial. j Quantification of the ATF4 5′UTR-dsRed reporter intensities normalized to corresponding GFP intensities from the same cells. Midline represents the mean value, with the top and bottom of the box representing the maximum and minimum values. Asterisks above the boxes represent statistical significances between mutant and control values calculated with the a two-tailed t-test with ***p < 0.0001 and *p < 0.01. n = 158, 157, 112, 104, 139, 131, 120, 148, and 137, respectively for each box from left to right. Please see Source Data Files for raw data.

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