Fig. 6: Characterization of 3-input multicellular programs.

Design and characterization of 3-input single-cell (single-lineage) programs operating in various lineages (a). Multicellular programs were composed by mixing two (b) or three (c) single-lineage programs. The inputs are represented by letters, a (blue) for aTc (expression of Bxb1 Integrase), b (orange) for arabinose (expression of integrase TP901-1), and c (purple) for benzoate (expression of Int5). Strains were mixed in equal proportions and sequentially induced three times for 16 h each, with different order of occurrences of inputs. The bar graph corresponds to the mean value of fluorescence intensity (F.I.) in arbitrary units (a.u) for each fluorescent channel (GFP, RFP, and BFP), with different and linear scales each. All experiments were performed in triplicate three times on three different days (data distribution in dot plots in Fig. S14). The error bars correspond to the ± standard deviation of the mean of the three different experiments performed in triplicate on three different days, and measured using a plate reader. The dotted line indicates the autofluorescence of negative control strain.