Fig. 3: Incorporation of nucleoside analogues T-705 and T-1105. | Nature Communications

Fig. 3: Incorporation of nucleoside analogues T-705 and T-1105.

From: Rapid incorporation of Favipiravir by the fast and permissive viral RNA polymerase complex results in SARS-CoV-2 lethal mutagenesis

Fig. 3: Incorporation of nucleoside analogues T-705 and T-1105.The alternative text for this image may have been generated using AI.

a Structures of GTP, T-705 and T-1105 in their ribonucleotide triphosphate forms. b Elongation of the PT10/20 substrate in the presence of various analogue concentrations and the absence of ATP or GTP. Red dots show analogue incorporation sites at the omitted nucleotide positions, incorporation of correct nucleotides are indicated by blue letters and GTP:U mismatches by red letters. c Left panel shows products obtained with 100 µM of each analogue after 5 min. Right panel shows time-course in the presence of 10 µM of each analogue and absence of GTP. Site assignments for the hairpin substrate are not shown due to drastic differences in RNA migration attributed to changes in residual RNA structure following analogue incorporation. Full time-course series at multiple analogue concentrations are shown in Supplementary Fig. 5b. Source data are provided as a Source Data file.

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