Fig. 4: Amino acid transporter Path is required for HSD-enhanced Ras/Src-tumour growth.
From: Systemic muscle wasting and coordinated tumour response drive tumourigenesis
a Relative log2fold change of SLC7- and SLC36-family amino acid transporters in dissected tumour tissue from ras1G12V;csk−/− animals raised on HSD compared to animals raised on CD, as determined by qPCR. Samples are normalised to Act88F. Results are shown as mean ± SEM. Data from n = 6 (Act88F), n = 6 (mnd), n = 6 (JhI-21), n = 4 (slif), n = 6 (CG8785), n = 6 (path) and n = 6 (CG1139) biologically independent samples. Data were analysed by two-tailed unpaired Student’s t test. Asterisks indicate statistically significant difference (**P < 0.01; ****P < 0.0001). Dissected eye epithelial tissue stained with DAPI (red) from ras1G12V;csk−/− animals raised on HSD (b), with mndRNAi/GD (c), JhI-21RNAi/GD (d), slifRNAi/GD (e), CG8785RNAi/GD (f), pathRNAi/KK (g) and CG1139RNAi/GD (h). Scale bar, 250 μm. Anti-Path staining (red) of dissected tumour tissue from ras1G12V;csk−/− animals raised on CD (i), ras1G12V;csk−/− animals raised on HSD (j), ras1G12V;csk−/−,wts animals raised on HSD (k) and ras1G12V;csk−/−,yki animals raised on CD (l) with DAPI (blue). Scale bar, 100 μm. Anti-CG1139 staining (red) of dissected tumour tissue from ras1G12V;csk−/−animals raised on CD (m), ras1G12V;csk−/− animals raised on HSD (n) and ras1G12V;csk−/−,wts animals raised on HSD (o), and ras1G12V;csk−/−,yki animals raised on CD (p) with DAPI (blue). Scale bar, 100 μm.
