Fig. 3: Specific interaction between the N6 amino group of −2A base and Cys27.
From: Elucidation of the aberrant 3′ splice site selection by cancer-associated mutations on the U2AF1
a Space-filling representation around the −2A base. The side chains of the U2AF1 amino-acid residues are shown in blue, and the RNA bases in orange. The interface around −2A base is intimately surrounded by the amino-acid residues of U2AF1. Thus, no space is found for the N6-methyl modification of the –2A base to interact with U2AF1. b Binding affinity of U2AF1 WT for the methyl modified RNA, 5′-UUm6AGGU, measured by ITC. Raw data and the corresponding binding curve are depicted. c RNA-binding activities calculated by ITC measurements. Mean value for the dissociation constant (Kd) with standard deviation is based on three independent measurements.
