Fig. 9: IL-33⁺ glioma associate with recruitment of peripheral immune cells.

a Pseudocolor plots and histograms shown are from representative sham, K1492-control, and K1492-IL-33 tumor-bearing mice. For gating strategy see also Supplementary Fig. 7. Histogram: red represents K1492-IL-33, blue represents K1492-control, dark gray represents sham, and light gray represents isotype control for respective fluorochrome shown. Lower left histogram shows the distribution of live CD45^low, CD45^int, and CD45^high cells within the leukocyte gate. Upper left histogram shows the distribution of cells within the CD45^high population that are CD11b⁺ (i.e., myeloid cells). Upper panels show distribution of cells in sham, K1492-control, and K1492-IL-33 glioma within the CD45^highCD11b⁺ gate that are Ly6G⁺ (neutrophils), Ly6G⁻Ly6C^high (inflammatory monocytes), and Ly6G⁻Ly6C^int activated monocytes. Lower panels show distribution of cells in sham, K1492-control, and K1492-IL-33 glioma, within the CD45^high gate that are CD3⁺NK1.1⁻ (CD3 T cells), CD3⁺ NK1.1⁺ (NKT cells), and CD3⁻NK1.1⁺ (NK cells). Plots on the far right show the distribution of CD45⁺CD11b⁺ cells that are CD45^low (microglia; blue), CD45^int (activated microglia; gray), and CD45^high (infiltrated myeloid cells; orange) in sham, K1492-control, and K1492-IL-33 glioma. Scatter plots show the number of cells as indicated on left axis, as isolated and quantified per tumor-bearing hemisphere in b sham, K1492-control, and K1492-IL-33 glioma, and c sham, U87-control, U87-IL-33, and U87-ΔNLS. In c, bottom far right scatter plot shows the proportion of cells that are CCR2⁺ or Ly6C⁺ amongst the CD11b⁺ cells that are CD45^low, CD45^int, or CD45^high. Each point represents data from a single mouse. The experiment was repeated three times with comparable results. Red line indicates the mean. NS not significant; *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA with Tukey’s post hoc test. See also Supplementary Fig. 7. Source data are available as a Source data file.