Fig. 3: PD-L1 on DCs is upregulated by IFN-γ and T cells in tumor microenvironment. | Nature Communications

Fig. 3: PD-L1 on DCs is upregulated by IFN-γ and T cells in tumor microenvironment.

From: PD-L1 on dendritic cells attenuates T cell activation and regulates response to immune checkpoint blockade

Fig. 3: PD-L1 on DCs is upregulated by IFN-γ and T cells in tumor microenvironment.The alternative text for this image may have been generated using AI.

a, b In naïve or MC38 tumor-bearing WT mice, PD-L1 levels on cDC1 (CD11b−CD24+) and cDC2 (CD11b+CD24−) were measured by flow cytometry (n = 7, except naïve spleen n = 6). ***p < 0.0001; *p = 0.0106; **p = 0.0030. c BMDCs were generated by FLT3-L. PD-L1 levels were measured by flow cytometry (n = 3). **p = 0.0010. d BMDCs were treated with recombinant IFN-α or IFN-γ. PD-L1 levels were measured 24 h later (n = 3). ***p(IFN-α) < 0.0001; ***p(IFN-γ) < 0.0001. e Purified WT DCs were treated with IFN-α or IFN-γ. PD-L1 levels were measured 24 h later (n = 3 mock, 4 IFN-α, 4 IFN-γ). **p(cDC1:IFN-α) = 0.0022; **p(cDC1:IFN-γ) = 0.0015; ***p(cDC2:IFN-α) < 0.0001; ***p(cDC2:IFN-γ) < 0.0001. f C57BL/6 mice (n = 7, except anti-IFNAR1 n = 6) were inoculated with 5 × 105 MC38-EGFP cells. Mice were treated with IgG, anti-IFNAR1, anti-IFN-γ, or anti-CD8. PD-L1 levels on cDC1 and cDC2 in tumor tissues were measured by flow cytometry on day 14 after inoculation. *p = 0.0208; ***p = 0.0005. g Purified WT DCs and activated CD8+ T cells were cocultured with or without anti-IFN-γ for 24 h. PD-L1 levels on cDC1 and cDC2 were measured by flow cytometry (n = 4, except DC + T n = 3). *p = 0.0442; **p = 0.0013. Data are shown as mean ± SEM and are representative of two independent experiments a, c–e, and g or pool of two independent experiments (b and f). n.s., not significant; *p < 0.05; **p < 0.01; ***p < 0.001 determined by unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.

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