Fig. 3: T-Plastin enhances protrusion dynamics.
From: T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migration
a HUVEC expressing F-tractin-mCitrine were treated with control or T-Plastin siRNA, sparsely plated, and imaged every 10 s for ~33 min. Masks were generated of the cell edge boundaries and time-projected with each line representing a 50 s interval, the parula colormap represents the 0 min time as blue and 33.3 min time as yellow. Straight yellow lines over F-tractin images indicate regions used to generate kymographs shown in (b). Bars, 10 µm. b Kymographs generated from F-tractin images shown in (a), bars 2.5 µm in the x-direction. The y-direction is time with each pixel as 10 s, the entire length equaling 33.3 min. Protrusions can be seen moving outward from the cell body (toward the right) and retractions inward toward the left. c Quantification of protrusion rates generated from kymographs such as those shown in (b). (n of protrusions are: siControl = 38, siT-Plastin #1 = 34, siT-Plastin #2 = 30, taken from two independent replicates). d Quantification of retraction rates generated from kymographs such as those shown in (b). (n of retractions are: siControl = 28, siT-Plastin #1 = 28, siT-Plastin #2 = 20, taken from two independent replicates). Bars represent means, error bars represent 95% CI, each siRNA targeting T-Plastin was compared to siControl using a one-way ANOVA with Dunnett’s multiple comparison test **P < 0.01, ***P < 0.001.
