Table 1 NMR and refinement statistics of the (DMA-135)-SLII complex.

NMR distance and dihedral constraints

Distance restraints

Total NOE

506

Intra-residue

162

Inter-residue

344

 Sequential (|i – j | = 1)

245

 Nonsequential (|i – j | > 1)

6

 Hydrogen bonds

75

 C^H RDCs (base pair only)

23

Q_free (%)

 Intermolecular (SLII to DMA-135)

29

 Total dihedral angle restraints

Nucleic acid

 Base pair

N/A

 Sugar pucker

155

 Backbone

N/A

 Based on A-form geometry

Structure statistics

Violations (mean and s.d.)

 Distance constraints (>0.4 Å)

1.30 ± 1.41

 Dihedral angle constraints (>5°)

N/A

 Max. dihedral angle violation (>5°)

N/A

 Max. distance constraint violation (Å)

0.40 ± 0.38

Deviations from idealized geometry

 Bond lengths (Å)

0.01112 ± 0.0007

 Bond angles (°)

2.495 ± 0.018

 Impropers (°)

N/A

Average pairwise r.m.s. deviation^a (Å)

RNA

 Heavy

3.26 ± 1.92

 Backbone^b

3.25 ± 2.08

 Upper helix (12–20 & 27–35)

 Lower helix (1–5 & 35–41)

 Complex

0.56 ± 0.11

 All complex heavy (C, N, O, P)^c

3.54 ± 1.86