Fig. 1: In situ modification of gut bacteria by oral delivery.
From: In situ reprogramming of gut bacteria by oral delivery
Temperate phage is engineered to express dCas9, trcrRNA, and crRNA so that specific bacterial genes can be repressed (a). The encapsulation strategy uses calcium-mediated crosslinking of a sodium alginate solution to produce beads, which are coated in a polymeric multilayer (b). The digestive system has longitudinal variations in pH27,40 and bacteria41, and presents multiple challenges to biological therapeutics during their transit through the stomach, small intestine, and large intestine (c).
