Fig. 3: ACKR3 stimulation impairs gap junctional electrical coupling between astrocytes.

a Representative membrane currents recorded in cell pairs of secondary astrocyte cultures (whole-cell configuration of the patch-clamp technique, holding potential −50 mV). Each chart recording illustrates the current recorded in the stimulated celI (I1, upper trace, in response to depolarizing voltage steps, 40 mV amplitude, 300 ms duration, every 30 s) and the junctional current recorded in the non-stimulated cell (I2, bottom trace). I1 and I2 were continuously monitored before and after the treatment of cells with either vehicle, CXCL12 (10 nM), CXCL11 (100 nM), or CBX (100 μM). b Time-course of the effect of CXCL12, CXCL11, and CBX on Ij magnitude. Perfusion of the recorded cell pairs with the different compounds started at the time indicated by the dotted line. Data are expressed as a percentage of Ij (normalized to the average ratio recorded during the first 4 min before any treatment). Values are means ± SEM (Two-way ANOVA, Bonferroni post-hoc, F(87,573)). c Ij before and after treatment for each cell pair recorded in the different conditions are plotted. Data are expressed as a percentage of Ij (normalized to the average ratio recorded during the first 4 min before any treatment, Two-tailed Wilcoxon test). See the Statistics and Reproducibility section for the number of repetitions, exact P values and symbol (*) legend. Source data are provided as a Source Data file.