Fig. 4: Driver mutations and clonality of Brca1-deficient breast tumors revealed by scWES. | Nature Communications

Fig. 4: Driver mutations and clonality of Brca1-deficient breast tumors revealed by scWES.

From: Characterization of BRCA1-deficient premalignant tissues and cancers identifies Plekha5 as a tumor metastasis suppressor

Fig. 4

a Number of nonsynonymous SNVs of human homologous genes in single cells and paired bulk samples from different tumor developmental stages. A big dot indicates a bulk sample, a small dot indicates a single cell (VMGB, n = 1 bulk tissue; VMMG, n = 17 cells; 476MGB, n = 1 bulk tissue; 476MMG, n = 9 cells; 476PTB, n = 1 bulk tissue; 476PT, n = 20 cells; 153PTB, n = 1 bulk tissue; 153PT, n = 18 cells; 153LMTB, n = 1 bulk tissue; 153LMT, n = 20 cells. Error bars represent SD). b Oncoplot summarizing recurrently altered genes in single cells and paired bulk samples according to the developmental stages. A gray rectangle indicates the wild-type status of the indicated site, a light gray rectangle indicates no coverage of the indicated site. c Incidence plot of frequently mutated genes (≥3 affected cells) detected in the series (n = 50 genes; n = 84 cells). d The neighbor-joining phylogenetic tree showing the clonality of 476MG and 476PT. The subclones were circled and determined by at least more than four cells distributed in a trunk. e The neighbor-joining phylogenetic tree showing the clonality of 153PT and 153LMT. The subclones were circled and determined by at least more than four cells distributed in a trunk.

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