Fig. 5: Fasn deletion in SGC does not alter neuronal functional properties.

a Quantification of cleaved caspase 3 in DRG sections from control and FasncKO mice in naïve and 3 days after sciatic nerve injury. The ratio of cleaved caspase 3 positive vs. DAPI nuclei was measured. n = 4 biologically independent animals. ns-non significant. b qPCR analysis of Atf3 expression in DRG from control and FasncKO mice in naïve and 3 days after sciatic nerve injury. n = 3 biologically independent animals. c Whole-cell recordings were performed from cultured DRG neurons associated with at least one glial cell, from FasncKO or control mice. Resting membrane potential (RMP) in control −45.3 ± 2.7 mV, n = 16 cells from 8 independent experiments; in FasncKO −46.1 ± 1.8 mV, n = 30; p = 0.79. d Membrane capacitance in control 18.3 ± 1.8 pF, n = 16 cells from 8 independent experiments; in FasncKO 16.5 ± 1.1 pF, n = 30 cells from 8 independent experiments; p = 0.36. e Input resistance in control 456 ± 43 MΩ, n = 16 cells from 8 independent experiments; in FasncKO 482 ± 24 MΩ, n = 30 cells from 8 independent experiments; p = 0.56. f Spiking properties of DRG neurons from controls and FasncKO. g Action Potential threshold in control −9.46 ± 0.94 mV, n = 13 cells from 8 independent experiments; in FasncKO −9.91 ± 0.84 mV, n = 28 cells from 8 independent experiments; p = 0.77 ns- non-significant. h Neuronal firing rate in control 11.57 ± 2.09 Hz, n = 14 cells from 8 independent experiments; in FasncKO 10.62 ± 0.86 Hz, n = 29 cells from 8 independent experiments; p = 0.62. ns- non significant. P values determined by One way ANOVA followed by Sidak’s multiple comparisons test in (a, b) and two-tailed t test in (c, d, e, g, h). Data are presented as mean values ± SEM. Source data are provided as a Source Data file for (a–e, g, h).