Fig. 6: Fasn deletion in SGC impairs axon regeneration.

a Representative longitudinal sections of sciatic nerve from control and FasncKO mice 3 days after sciatic nerve injury, stained for SCG10. Orange lines indicate the crush site, identified as the maximal SCG10 intensity. Arrowheads indicate longest regenerating axons. Scale bar: 500 µm. b Length of the longest 10 axons was measured in 10 sections for each nerve. c Regeneration index was measured as SGC10 intensity normalized to the crush site. d Representative images of dissociated DRG neurons from control and FasncKO, cultured for 20 h, from naïve and injured (3 days post conditioning sciatic nerve injury) and stained with the neuronal marker TUJ1. Scale bar: 200 µm. e The length of axons per neuron was measured. Automated neurite tracing analysis using Nikon Elements of about 500 neurons per replicate. f Percentage of initiating neurons normalized to the total number of neurons was measured in 8 independent animals, average of 500 neurons per replicate. Automated neurite tracing analysis using Nikon Elements. One way ANOVA. Sidak’s multiple comparisons test ns-non-significant. n = 8 biologically independent animals examined over 2 independent experiments for (a–f). P values determined by two tailed t test presented in (b) and One way ANOVA followed by Sidak’s multiple comparisons test in (c, e and f). Data are presented as mean values ± SEM. The boxplots in b and e extends from the 25th to 75th percentiles and the whiskers go down to the smallest value and up to the largest where the box middle line is the median. Source data are provided as a Source Data file for (b, c, e, f).