Fig. 6: Cooperation between STAT5 and BATF converts Th17 into proallergic cells.

a–g Naive CD4+ T cells were isolated from OTII (CD45.2) mice and differentiated into Th17 cells for 5 days. Retrovirus expressing caSTAT5 (tagged with hNGFR) and/or BATF (tagged with Thy1.1) was transduced on day 1. Transduced cells were sorted by gating on reporter-positive and CD4-positive cells before transfer to Boy/J (CD45.1) mice. Recipient mice were challenged daily for 6 days with intranasal OVA. One day after the final challenge, mice were analyzed for inflammation of lung tissue by H&E and PAS staining (b), IL-9 concentration in BALF (c), neutrophil, eosinophil, and mast cell numbers (d–f), and serum concentration of MCPT-1 (g) (n = 4 per group). h caSTAT5- and BATF retrovirus-transduced OTII Th17 cells were transferred to Boy/J mice followed by 6 days OVA challenge and anti-IL-9 or isotype antibody were given 5 h before each OVA challenge. BALF cells were counted. Lung eosinophil and mast cells were analyzed by FACS. Serum MCPT-1 was detected by ELISA (n = 3 per group). Data are mean ± SEM. Two-way ANOVA with Sidak’s multiple comparison test was used to generate p-values in c–g. An unpaired two-tailed Student’s t-test was used for comparisons in h. See also Supplementary Fig. 5.